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RINF maintenance of SMAD7 sustains human erythropoiesis
SMAD7 promoter (Figure 5B). As shown in Online Supplementary Figure S5C, we found that RINF binds direct- ly to several regions (R1 to R4) of the SMAD7 promoter and that this binding was increased in cells overexpressing RINF. Moreover, H3K4me3 histone marks, quantified by
AB
ChIP-qPCR (Figure 5C), were increased by RINF overex- pression in four proximal regions (R2-R4) of the SMAD7 transcription start site, indicating that chromatin was in a more active transcriptional state.
To investigate functional relationships between RINF
CD
EF
Figure 4. Legend on following page.
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