Page 143 - 2021_03-Haematologica-web

Iron deficiency and thrombosis
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Figure 5. Iron deficiency enhances arterial thrombotic tendency.
Arterial thrombosis was induced in animals fed an iron-deficient diet for 7 weeks and given three injections of ferric carboxymaltose at a dose of 20 mg/kg body weight or placebo (0.9% NaCl). (A) Hemoglobin concentration and platelet counts prior to surgery (n=9-11 per group). (B) Thrombus area and length measured on Carstairs-stained longitudinal sections of thrombi (n=5-7 per group). (C) Representative images of Carstairs-stained arterial thrombi. (D) Thrombus area versus platelet count (n=5-7 per group). (E) Time until cessation of carotid blood flow due to throm- bus development (n=9-11 per group). (F) Area of thrombus stain- ing positive for ITGA2B (n=5 per group). (G) ITGA2B-positive area versus platelet count (n=5 per group). (H) Representative images of ITGA2B staining of arterial thrombi. *P<0.05, **P<0.01, ***P<0.001. Error bars: mean ± standard deviation. Hb: hemoglo- bin; PLT: platelet count. Con: animals fed a normal diet and given placebo injections; Def: animals fed an iron-deficient diet; Def+Fe: animals fed an iron-deficient diet and then given injections of ferric carboxymaltose.
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