Page 239 - Haematologica May 2020
P. 239

IL6R-STAT3-ADAR1(P150) interplay in 1q21(amp) MM
     E
F
B
A
CD
Figure 5. Combined IL6R and P150 suppression led to reduced IL6-induced oncogenicity. (A) The association between 1q21 status and STAT3 signature in Dana Farber Cancer Institute (DFCI) and University for Arkansas Medical School (UAMS) patient’s datasets. WT- wildtype, Amp- two or more copies. (B) CTG assay assessing the growth rate of multiple myeloma cell lines with different 1q21 status over a 72 h time course. **P<0.05, ***P<0.001 (C) At 48 h after lentivirus infection, U266 cells with shRNA- mediated P150 and/or IL6R knockdown were treated with IL6 (10 ng/mL) for 8 h and then the STAT3 pathway protein expression profile was analyzed by western blotting. (D-F) Daily growth assays (D), colony formation assays (E) and cell cycle assays (F) were done 48 h after lentivirus infection of shRNA to elucidate the phenotypes associated with loss of either one or both P150 and IL6R. For the cell cycle assays, the cells were stimulated with IL6 (10 ng/mL) for 24 h (to induce STAT3 signaling) before being fixed for fluorescence activated cell sorting analysis. This was to study the direct effects of loss of expression of these genes on the STAT3-mediated cell cycle profile. **P<0.05 against shCtr, ***P<0.001 against shCtr, ###P<0.001 against shP150, ^^P<0.05 against shIL6R.
   haematologica | 2020; 105(5)
1399
   

























































































   237   238   239   240   241