Page 245 - Haematologica March 2020

SOX11 and TP53 status in MCL
were in the negative/low SOX11 group (RNAscope 0-1) (9/15; 60%) and four in the high SOX11 group (RNAscope 3-4) (4/36; 11%). This difference was statisti- cally significant (P=0.0007). Within the group of cMCL four TP53 mutated cases where in the low SOX11 group (4/7; 57%) and four in the high SOX11 group (4/36; 11%), and the difference was statistically significant (P=0.01) Of the cMCL, the TP53 mutated cases showed the lowest
SOX11 mRNA scores including two cases with blastoid morphology (Figure 4).
Discussion
In this study, we investigated 66 cases of MCL for the expression levels of SOX11 mRNA with RNAscope ISH
AB
E
F
CD
Figure 3. Classical cyclin D1 negative mantle cell lymphoma (D1-MCL) with mantle zone pattern (case #10). (A) the architecture is characterized by the expansion of the follicle mantle zone with tumor cells and a central “naked” germinal center (hematoxylin and eosin), (B) CD20 highlights the expanded mantle area; (C) MIB1 shows a high proliferation in the residual germinal center whereas the proliferation of the tumor cells is 5%; (D) the tumor cells are negative for cyclin D1; (E) the SOX11 stain demonstrates a strong positivity in the tumor cells, while the germinal center cells are negative; (F) the SOX11 RNA in situ hybridization (RNAscope) shows a score 4 with 15 dots/cell and >10% cells with dot clusters. All original magnification 200x.
haematologica | 2020; 105(3)
759