Page 244 - Haematologica March 2020

B. Federmann et al.
Table 2. SOX11 RNAscope and RT-qPCR analyses in the three MCL subgroups.
Diagnosis
SOX11 mRNA level by RNAscope and RT-qPCR#
Score 0 RT-qPCR median (range)
Score 1
RT-qPCR median (range)
Score 2
RT-qPCR median (range)
Score 3
RT-qPCR median (range)
Score 4
RT-qPCR median (range)
cMCL 49 0 - 7 7(4-68) 11 15(2-43) 13 24(6-119) 18 39(3-218)
D1-MCL 6 0 - 0 - 1 8.63 1 7.57 4 59+
nnMCL 8 7 1(0-1) 1 1 0 - 0 - 0 -
Total 63 7 1 (0-1) 8 7 (1-68) 12 10 (2-43) 14 23 (6-119) 22 39 (3 -218)
cMCL: conventional mantle cell lymphoma; D1-MCL: cyclinD1 negative mantle cell lymphoma; nnMCL: non-nodal leukemic mantle cell lymphoma; real-time quantitative reverse transcription-PCR. #54 cases could be analyzed with RT-qPCR. + mean of the two cases analyzed with RT-qPCR.
AB
CDE
F
G
Figure 2. Mantle cell lymphoma (MCL) with splenic involvement and plasmacytoid differentiation (case #9). (A) the spleen is characterized by a nodular infiltration of the white pulp (hematoxylin and eosin); (B) Giemsa stain highlights the cytologic features. The infiltrate is composed of medium-sized cells with a rim of basophilic cytoplasm, dispersed nuclear chromatin and occasional small nucleoli; (C) the cells are positive for cyclin D1; (D) the tumor cells are positive for lambda-stain (D) and negative for kappa (E); (F) the SOX11 immunohistochemistry (IHC) was interpreted as negative at diagnosis, but upon review occasional weakly positive tumor cells could be detected; (G) the SOX11 RNA in situ hybridization (RNAscope) shows a score 1 with 1-3 dots/cell. A original magnification 50x, B-G original magnifi- cation 200x, insert original magnification 630x.
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