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Low TLT-1 and collagen receptor α2 in FPD/AML
Figure 4. Levels of TLT-1 in megakaryocytes (MK) in familial platelet disorder with predisposition to acute myelogenous leukemia. Double immunofluorescenc labeling of TLT-1 and von Willebrand factor (vWF) in mature MK grown from peripheral blood CD34+ cells from one control and 2 patients (DII-1 and DIII-3) Representative confocal microscopy images of sorted CD41+D42+ cells stained with rabbit anti-TLT-1, mouse anti-vWF, Phallodin-Alexa 633 and 4′,6-diamidino-2 phenylindole. Anti-rabbit Alexa-488 and anti-mouse Alexa-546 were used as secondary antibodies. White arrows indicate staining for TLT-1 in granules that do no contain vWF.
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