Page 44 - 2018_10-Haematologica-web

V. Lapostolle et al.
A
B
Figure 5. CD133 expression by CD34+ cells selected on the basis of CXCR4 expression. Three gates were delimited: P1, CD34+CXCR4neg cell subpopulation; P2, CD34+CXCR4low cell subpopulation; P3, CD34+CXCR4bright cell subpopulation. Among these cell gates, CD133+ and CD133- fractions were defined and six cell subpop-
sorted as CXCR4negCD133-,
ulations were
CXCR4negCD133+, CXCR4lowCD133-, CXCR4lowCD133+, CXCR4brightCD133-, and CXCR4brightCD133+ subfractions. For each of these subpopulations, the percent of CD133-expressing cells is indicated.
AB
Figure 6. CD133 determines hematopoietic severe combined immunodeficiency repopulating cell capacity of CXCR4-expressing CD34+ steady-state peripheral blood cells, before and after ex vivo expansion. Severe combined immunodeficiency repopulating cell (SRC) activity was evaluated by short-term engraftment (8 weeks) in NSG mice. Each point of the graphs represents the percentage of CD45+ human cells in one mouse bone marrow. (A) Day 0: 1x105 SS-PB total CD34+ cells or 1x105 cells from each sorted cell subpopulation were injected per mouse. (B) Day 7: 2x105 of the total expanded cell population or 2x105 cells from each sorted cell subpopulation were injected per mouse. The “pie” graphs in (A) and (B) show the relative proportions of CD19+ and CD33+ cells pf human origin within the huCD45+ population. Statistical significance: **P<0.01 and ***P<0.001.
1610
haematologica | 2018; 103(10)