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L. Doll et al.
Consistent with a previous study,22 an average 1.7-fold increase of mpo+ cells was observed when g-csfa was ectopically expressed in wild-type embryos (Figure 6E). Next, the g-csfa inducible construct was co-injected with hax1 e1-MO into embryos. Heat shock was performed at
1 dpf and GFP+ embryos were selected for WISH analysis. As expected, embryos injected with e1-MO displayed reduced neutrophil numbers. However, when g-csfa was induced in the morphants, we observed that the number of neutrophils was increased to a level which was compa-
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Figure 4. Normal development of erythrocytes, macrophages and hematopoietic stem cells in the hax1 morphants. (A-H) Left panels show representative images of whole mount in situ hybridization for gata1 (A), hbae1.1 (B), pu.1 (C), l-plastin (D), lyz (E), g-csfr (F), mpeg1.1 (G) and cmyb (H) expression in uninjected (WT) and hax1 e1-MO injected embryos at 24 hours post-fertilization (hpf) (A,B) or 48 hpf (C-H). Right panels show quantitative numbers of stained cells in the trunk region. Scale bars indicate 100 mm. Each dot in C-G represents an individual embryo. Data are means ± standard deviation.
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haematologica | 2021; 106(5)