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Pro-leukemic effects of P2X7
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Figure 5. MA9-P2X7 cells have increased leukemia stem cells. (A) GFP+BFP+ leukemia cells were sorted and seeded onto 24-well plates (500 cells/well) for colony- forming assays. Typical Giemsa-stained dishes are shown (left panel), and colony numbers are plotted (right panel). (B) Different numbers of sorted GFP+BFP+ leukemia cells (1×104 and 1×103 for MA9 cells; 1×104, 1×103 and 1×102 for MA9-P2X7 cells) were transplanted into recipient mice, and the survival of mice was recorded. (C) The frequency of leukemia stem cells (LSC) was calculated using L-calc software. (D) Leukemia cells were stained with c-Kit. The gating strategy is shown (left panel), and the percentage of c-Kit+ cells is plotted (right panel). (E) Sorted MA9 c-Kit–, MA9 c-Kit+ and MA9-P2X7 cells were transplanted into recipient mice. The percentage of c-Kit+ leukemia cells was analyzed at the late stage of leukemia. (F) The same numbers of MA9, MA9 c-Kit–, MA9 c-Kit+ and MA9-P2X7 leukemia cells were transplanted into recipient mice, and survival was recorded. Bars represent the mean ± standard error of mean. *P<0.05; **P<0.01; ***P<0.001; unpaired Student t test and one-way analysis of variance. 95% CI: 95% confidence interval.
haematologica | 2021; 106(5)
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