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HLA-G corrects dysfunction of immune cells in ITP
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Figure 2. Impaired expression of immunoglobulin-like transcript in immune thrombocytopenia patients was recovered with exposure to rhHLA-G. (A) Cell surface expres- sion of immunoglobulin-like transcript 2 (ILT2) on CD4+, CD8+, CD14+, and CD19+ cells and ILT4 on CD14+ cells in immune thrombocytopenia (ITP) patients (n=17) and healthy controls (n=15) after culture with or without recombinant human leukocyte antigen-G (rhHLA-G) (600 ng/mL, 3 days). *P<0.05; **P<0.01. (B) Representative his- tograms of isotype control and ILT2 on CD4+, CD8+, CD14+, and CD19+ cells and ILT4 on CD14+ cells in an ITP patient. Representative scattergrams of surface expression of CD14, CD4, CD8, CD19 from an ITP patient. Side scatter (SSC)lowCD14+, SSClowCD4+, SSClowCD8+, SSClowCD19+ cells were gated for the following analysis.
timulatory molecules, such as CD80, CD86 and related cytokines, could contribute to T-cell proliferation and differentiation. In order to further evaluate DC-mediated T-cell proliferation, allogeneic CD4+ T cells were cocul- tured with DC with or without rhHLA-G premodula- tion. DC from ITP patients stimulated significantly more CD4+ T-cell proliferation compared with those from healthy controls in vitro. However, rhHLA-G-modulated DC from ITP patients lost their suppressive effect on CD4+ T-cell proliferation compared to unmodulated cells (Figure 5E-F).
HLA-G treatment alleviated thrombocytopenia in a murine model of ITP
HLA-G capture by gold nanoparticle was observed by transmission electron microscopy (Figure 6A). The SCID mice engrafted with splenocytes from immunized CD61 KO mice exhibited profound thrombocytopenia within 28 days after transplantation. The SCID mice were separated into four groups. Group 1 received an intraperitoneal injection of 100 mL AuNP coated with 7.5ug HLA-G on the day of the splenocyte transplantation; group 2 received an intraperitoneal injection of same amount of control AuNP
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