Page 141 - Haematologica Vol. 110 - January 2025

ARTICLE - Immune microenvironment in nodal PTCL P. Stephan et al. A
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Figure 2. Diverse phenotypes of CD4+ conventional T cells in peripheral T-cell lymphoma. (A-D) Unsupervised clustering analyses following manual gating on live CD3+CD4+Foxp3- conventional T cells (Tconv). (A) UMAP visualization, FlowSOM distribution of clusters and projection of selected markers in concatenated samples after random selection of an equal number of cells between each group. (B) Heatmap showing hierarchical clustering and expression (normalized across markers) of indicated markers in FlowSOM clusters, and their differential enrichment between groups. (C) Distribution of cells from each group on UMAP. (D) Pro- portion of PD-1 and ICOS-expressing Tconv cells upon manual gating. Mean + Standard Error of Mean is shown. Two-way ANOVA tests were used; only the comparison of PD-1+ICOS+ cells between groups is shown. TFH: follicular helper cells; FC: flow cytom- etry; FDR: false discovery rate; LN: lymph nodes; AITL: angioimmunoblastic T-cell lymphoma; PTCL, NOS: peripheral T-cell lym- phoma, not otherwise specified. *P<0.05, ***P<0.001.
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