Page 99 - 2020_08-Haematologica-web

The critical function of Tfr1 in hematopoiesis
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Figure 3. Neonatal Tfr1 conditional knockout mice have defects in multiple cell lineages. (A) Gating strategy (left panel) and the absolute number of erythrocytes (right panel) at the indicated differentiation stages measured in the bone marrow of control and Tfr1 knockout (cKO) mice at P3 (n=4 per group). (B, C) Gating strategy (left panel) and absolute numbers (right panel) of Mac1+Gr1+ myeloid cells (B) and B220+ B cells (C) in the spleen and liver of control and cKO mice (n=4 for each group). (D) Total number of cells in the thymus. (E) Cells were gated according to CD4 and CD8 expression (left panel), and the absolute cell numbers are shown at the right. (F) H&E-stained bone marrow sections obtained from a control and cKO mouse at P3. (G) Total number of cells in the bone marrow of control and cKO mice at P3 (n=4 for each group). (H) Absolute numbers of LSK (Lin-cKit+Sca1hi) and hematopoietc progenitor cells (HPC )(Lin-cKit+Sca1–) cells in the bone marrow of control and cKO mice at P3 (n = 4 for each group). (I) Percentage of Annexin V‒positive LSK cells in control and cKO mice (n=4 for each group). *P<0.05; **P<0.01; ***P<0.001.
haematologica | 2020; 105(8)
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