Page 177 - 2020_08-Haematologica-web
P. 177
Apoptosis induced by selective BH3-mimetics
haematologica | 2020; 105(8)
2153
Table 1. Characteristics of the cell lines.
Cell line
Gene expr. subtype
Genetic driver subtype
Driver mutations
BCL2 family mutations
S63845 EC50
A1210477 Original EC50 ref.
Mutation ref.
RIVA/Ri-1
ABC
Other ABC
BCL2 amp, PMAIP1 amp
0,008
1,127 7,500
5,500>10
>10 40
31
U2932
OCI-LY1
SUDHL4 GC EZB EZH2 t(14;18) BCL2
1,439 0,245 5,000 5,625 >10
4,520 ND 6,0390 ND 2,224 ND
5,260 0,170 0,470
5,466 41 >10 42 >10 43 >10 42 >10 44
31 Cosmic Cosmic CCLE Cosmic
OCI-LY10 ABC
MCD
5,740
ND 2,320
RC-K8 GC
BN2
BCL6 translocation, RELN,
0,0006
0,005 4,870
SUDHL8 GC
Other GC
KMT2D, CREBBP, EP300, SOCS1
6,170
0,005
0,012 0,530
>10 43
Cosmic
ABC GC
Other ABC EZB
EZH2, CREBBP, KMT2D CARD11, CD79a, MYD88
BCL2 amp, PMAIP1 amp t(14;18)
BCL2 BCL2 (silent)
MedB1 PMBL
SUDHL6 GC
TMD8 ABC
NU-DUL-1 GC
U2946 GC
SUDHL10 GC EZB Karpas-1106 PMBL EZB
SOCS1
EZH2, KMT2D, CREBBP, RELN CD79b, MYD88, PIM1 KMT2D
>10 3,016 1,635 9,846 >10 >10 >10
0,002 >10 3,598 3,603 >10 >10 >10
0,067 >10 >10 0,160 >10 0,330 ND 0,320 ND 0,140 >10 0,006 ND >10
>10 16 >10 43 4,253 45 6,973 46 >10 47 1,723 43 >10 17
Cosmic
SUDHL2 ABC HBL-1 ABC OCI-LY3 ABC
BN2 MCD MCD
NFKBIE, RELT TNFAIP3, MYD88 , EP300 CD79b, MYD88 CARD11, MYD88 , HLA-A , IRF4,
BCL2 amp
BCL2 (silent)
2,740 8,742 4,127
1,824 >10 6,653
ND not calc 4,000 5,900 ND 1,850
3,666 48 >10 49 >10 42
Cosmic Cosmic CCLE
Pfeiffer GC EZB
EZH2, KMT2D
t(14;18)
BIM, BCL2 (silent)
>10
>10
ND 3,430
>10 50
Cosmic
Other GC EZB MCD Other GC Other GC
t(14;18)
BCL2
Cosmic Unpublished Cosmic 27 Cosmic Cosmic
TNFAIP3, SPEN
EZH2, PTEN, EP300
MCL1 amp t(14;18)
EZH2, KMT2D, TNFAIP3,
PIM1, PRDM1
Genetic modifications Alterations
of BCL2 genes
Targeting BCL-2 ABT-199 EC50
Targeting BCL-XL A1331852 A1155463
Targeting MCL-1
Characteristics of diffuse large B-cell lymphoma cell (DLBCL) lines are displayed including the subtype of DLBCL and genetic modifications affecting the BCL-2 proteins.The EC50 values for the five BH3-mimetics used in this study are shown as calculated from the CellTiterGlo data displayed in Figure 1B-D and Online Supplementary Figure S1A, B (in mM). ABC: activated B-cell; GC: germinal center B cell; PMBL: primary mediastinal B-cell-like; MCD: MYD88 and CD79b mutations; BN2: BCL6 fusion and NOTCH2 mutations; EZB: EZH2 mutations and BCL2 translocations); ref: reference; EC50: half maximal effective concentration.
EC50 EC50
