Page 208 - Haematologica - Vol. 105 n. 6 - June 2020

S. Stivala et al.
including cryo-ET and super-resolution microscopy, show that upon high-shear conditions platelet GpIb receptors reorganize into “clusters” of 10 to 20 complexes closer to each other than on resting platelets; functionally, we demonstrate that, through intervention with the plant- derived n-3 FA ALA, the size of these clusters is decreased, thereby reducing platelet adhesion to vWF under high- shear flow. This highlights the potential of ALA as an anti- thrombotic agent.
Acknowledgments
This work was supported by a grant from the Swiss National Foundation of Science, the Swiss Heart Foundation, the Kardio Foundation, Switzerland, and the Budai Foundation, Lichtenstein, to JHB (grant n. 310030_144152), and the Mäxi Foundation to OM. We are grateful to Prof. B. Coller, New York, for the generous gift of the 6D1 antibody against human GpIb. We also thank the Center for Microscopy and Image Analysis at the University of Zurich (ZMB). Switzerland.
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