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Intronic deletion in MKL1 alters B-cell function
    HL1, and HL2 cells displayed similar expression of total surface and intracellular CD11a, and inactive CD11a (Figure 3C-G and Online Supplementary Figure S5C). HL0 cells displayed lower expression of total surface and intra- cellular CD11a, and inactive CD11a as well as lower CD11a mRNA (ITGAL) (Figure 3C-G and Online
A
Supplementary Figure S5C). Interestingly, cells lacking MKL1 expression19 showed higher surface CD11a expres- sion when compared to control cells (Online Supplementary Figure S4C). LCL from the triplet and con- trols had similar expression of CD54 (Figure 3H and Online Supplementary Figure S5C). This shows that HL0
 B
C
DEF
GH
Figure 3. HL0 cells display decreased aggregation and reduced CD11a integrin expression. (A) Aggregation of lymphoblastoid cell lines (LCL), representative images. (B) Average area of aggregates observed in (A). (C) Representative flow cytometry plots of total CD11a surface expression in LCL. (D) Quantification of CD11a surface expression as seen in (C). (E, F) Quantification of inactive CD11a surface expression and intracellular CD11a expression as seen in Online Supplementary Figure S3. (G) Expression of ITGAL (CD11a) by real-time quantitative polymerase chain reaction. (H) Quantification of CD54 surface expressio. (B, D-F) Combined data from three experiments. (G-H) Combined data from two experiments. For bar graphs, the dotted line indicates normalization to the mean of C1 and C2. All panels display data from LCL. Error bars represent the standard deviation of the measurements. Symbols represent technical replicates from three independent experiments in (G), and single values from three independent experiments in (F). All data were analyzed using analysis of variance with the post-hoc Tukey test. *P<0.05, **P<0.01, ***P<0.001.
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