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B. Nowlan et al.
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Figure 3. CD48 ligand CD244 is poorly expressed in the bone marrow of NOD-scid and NSG mice. (A) Viable single cells were gated into lymphoid (CD11b- low side scatter) and myeloid (CD11b+) cells. (B) Myeloid cells were further separated using CD169 and F4/80 antigens. Monocytes were CD11b+ F4/80+ CD169-; macrophages CD11b+ F4/80+ CD169+; and neutrophils and remaining myeloid progenitors were CD11b+ F4/80- CD169-. (C) CD244 expression was measured in each subset in each mouse strain and plotted as numbers of CD244+ cells per femur. (D) Lymphoid cells were separated using B220 and NK1.1 antigens to identify B cells (CD11b- B220+ NK1.1-) and natural killer (NK) cells (CD11b- NK1.1+). (E) The B220- NK1.1- gate was then plotted for CD3ε expression to identify T cells (CD11b- B220- NK1.1- CD3ε+). (F) CD244 expression on lymphoid subsets in each mouse strain. Numbers of CD244+ cells in each subset per femur. Data are mean ± standard deviation of five mice per group. P values were calculated by analysis of variance with Tukey corrections for multiple comparisons: **P≤0.01, ***P≤0.001, ****P≤0.0001.
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haematologica | 2020; 105(1)