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Figure 5. Competitive repopulation unit cells are enriched in the Lin- KIT+ CD27+ CD201+ FLT3- CD48- CD150+ population in NSG mice. (A) Transplantation outline using sorted male donor cells transplanted into sublethally irradiated (2.5 Gy) female NSG mice to test competitive repopulation unit (CRU) activity. (B-H) Sorting profile from donor male c-KIT enriched bone marrow into either the Lin- KIT+ CD27+ CD201+ FLT3- CD48- CD150+ gate or the Lin- KIT+ CD27+ CD201+ FLT3- NOT GATE. (I) Frequencies of donor engraftment in blood at 18 weeks. (J) Time-course of male donor cell engraftment in each transplanted mouse. Warm colors are for dilutions of sorted Lin- KIT+ CD27+ CD201+ FLT3- CD48- CD150+ cells, cold colors are dilutions of Lin- KIT+ CD27+ CD201+ FLT3- NOT GATE sorted cells. (J) Frequency of donor engraftment at 18 weeks post-transplantation and Poisson distribution of donor hematopoietic stem cells. tx: transplant.
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