Page 87 - 2020_01-Haematologica-web

CD27, CD201, FLT3, CD48, and CD150 identify HSC in mice
A
B
C
DF
E
Figure 4. CD48 ligand CD244 is poorly expressed in the spleen of NOD-scid and NSG mice. (A) Viable single cells were gated with CD11b into lymphoid cells (CD11b- low side scatter) and myeloid cells (CD11b+). (B) Myeloid cells were further separated using CD169 and F4/80 antigens. Monocytes were CD11b+ F4/80+ CD169-; macrophages CD11b+ F4/80+ CD169+; and neutrophils and remaining myeloid progenitors were in the CD11b+ F4/80- CD169- gate . (C) CD244 expression was meas- ured in each subset in each mouse strain and plotted as numbers of CD244+ cells per femur. (D) Lymphoid cells were separated using B220 and NK1.1 antigens to identify B cells (CD11b- B220+ NK1.1-) and natural killer (NK) cells (CD11b- NK1.1+). (E) The B220- NK1.1- gate was then plotted for CD3ε expression to identify T cells (CD11b- B220- NK1.1- CD3ε+). (F) CD244 expression on lymphoid subsets in each mouse strain. Numbers of CD244+ cells in each subset per femur. Data are average ± standard deviation of five mice per group. P-values were calculated by analysis of variance with Tukey corrections for multiple comparisons, *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.
haematologica | 2020; 105(1)
77