Page 84 - 2020_01-Haematologica-web

B. Nowlan et al.
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Figure 1. CD27 and CD201 expression in Lin- KIT+ bone marrow hematopoietic stem and progenitor cells from different NOD-scid-derived mouse strains. (A-C) Expression of CD27 and CD201 in viable Lin- KIT+ bone marrow (BM) hematopoietic stem and progenitor cells from C57BL/6 (A), NOD-scid (B) and NSG (C) mice. (D) Frequency of Lin- KIT+ CD27+ CD201+ cells within BM leukocytes of the three mouse strains. (E) Absolute number of Lin- KIT+ CD27+ CD201+ cells per femur in the three strains. (F-H) Comparison of SCA1 and KIT expression in Lin- KIT+ CD27+ CD201+ cells (red overlay) compared to Lin- KIT+ CD27+ CD201- cells (blue overlay) in C57BL/6 (F), NOD-scid (G) and NSG (H) mice. (I) Overlay of SCA1 expression in Lin- KIT+ CD27+ CD201+ cells from C57BL/6 (black), NOD-scid (blue) and NSG (red) mice. (J) Percentage of Lin- KIT+ CD27+ CD201+ cells that are SCA1+. (K) Number of Lin- CD27+ CD201+ cells that are SCA1+ per femur. Data are the mean ± standard deviation of five mice per group. P values were calculated by analysis of variance with Tukey corrections, *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.
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haematologica | 2020; 105(1)