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A. Uchida et al.
A Figure 3. Growth inhibitory effect and apoptotic sensitivity of the four cell lines to JQ-1 and three BH3 mimetics. (A) JQ-1 suppressed proliferation in three cell lines, but not in BJAB, in a dose-dependent man- ner. Venetoclax inhibited proliferation of Karpas231 and OCI-Ly8 cells even at a concentration of 20 nM, but had no effect on either BJAB or SU-DHL10 cells. S63845 showed the inhibitory effect only in SU- DHL10 cells. At nanomolar concentra- tions, A-1155463 failed to suppress the proliferation of any cell lines. (B) Although 50 μM of JQ-1 suppressed cell prolifera- tion in the SU-DHL10, Karpas231, and OCI-Ly8 lines, the exposure was insuffi- cient to induce apoptosis in Karpas231 (annexin V+ 7-aminoactinomycin D+ 30.3%) and OCI-Ly8 (annexin V+ 7-aminoactino- mycin D+ 4.4%) cells. Exposure to 200 nM of venetoclax effectively led to apoptotic changes (annexin V+) in more than 80% of both Karpas231 and OCI-Ly8 cells. Exposure to 100 nM of S63845 induced cell death only in SU-DHL10 cells. Consistent with the results in cell prolifera- tion assays, 1 μM of A-1155463 did not induce even modest cell death in any cell
lines. DMSO: dimethyl sulfoxide.
B
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haematologica | 2019; 104(7)