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S. Recalcati et al.
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Figure 5. Wound-infiltrating leukocytes and macrophage polarization are not altered in Fpn1fl/flLysCre+/- mice. (A) Frequencies of neutrophils (CD11b+/Ly6G+), eosinophils (CD11b+/CCR3+), T lymphocytes (CD3+), macrophages (CD11b+/F4/80+) on CD45+ cells. Frequencies of M1 (MHCII+/CD206-) and M2 (MHCII-/CD206+) polarized macrophages on total live macrophages. Dots and black lines represent single animals and the mean ± SEM, respectively; n=10 mice for each group; ***P<0.0001, **P<0.001, *P<0.01). (B) Bone marrow-derived macrophages from Fpn1fl/flLysCre-/- and Fpn1fl/flLysCre+/- mice were polarized to M1 and M2 macrophages and relative iNOS, TNFα, Arg1, YM1, FPN, TfR1 and CD163 mRNA levels were measured by quantitative real-time polymerase chain reaction at 24 h; results were normalized to the housekeeping gene 18S RNA (mean ± SEM of 12 mice for each group; ***P<0.0001, **P<0.001, *P<0.01).
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haematologica | 2019; 104(1)