Page 190 - 2022_03-Haematologica-web

Letters to the Editor
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Figure 2. Effects of zoledronic acid on multiple myeloma tumor growth upon immobilization. Mice were subjected to sham-operation (control), sciatic dener- vation (DN) or casting with adhesive bandages (CAST) of the right hind legs. Zoledronic acid (ZOL, 120 mg/kg) or saline were subcutaneously injected twice a week. Two weeks later, the right tibiae were taken out, and histomorphometrically analyzed. (A) Bone volume over total volume (BV/TV), trabecular thickness (Tb.Th), trabecular numbers (Tb.N) and trabecular separation (Tb.Sp) were assessed in trabecular bones. Data are expressed as the mean ± standard deviation (SD) (n=6). (B) TRAP staining was performed in the resected tibiae. Representative results were shown (magnification, ×100) (upper). Bars: 200 mm. Higher magnification (×400) of the boxed areas in the upper panels were shown in the lower panels. Bars: 50 mm. (C) Numbers of osteoclasts (N.Oc)/bone surface (BS) (/mm) were counted. Data are expressed as the mean ± SD (n=6). **P<0.01. (D) 5TGM1/luc multiple myeloma (MM) cells were inoculated into the right tibiae 2 weeks after DN. The mice were subcutaneously injected with 120 mg/kg zoledronic acid (ZOL) or saline twice a week. IVIS images were taken 4 weeks after the inoculation. Control groups were given saline as a vehicle. For IVIS imaging, 100 mL of 15 mg/mL D-luciferin in phosphate-buffered saline was injected intraperitoneally to mice before taking images. Five minutes after D-luciferin injection, the mice were placed in the imager with the mice anesthetized with 2% isoflurane while IVIS images were taken. Tumor areas with luminescence shown in green, yellow and red were measured. Px: pixel. Data are expressed as the mean ± SD (n=5). **P<0.01.
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haematologica | 2022; 107(3)