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ZBP1 regulates myeloma cell proliferation via IRF3/IRF4
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Figure 3. ZBP1 is required for myeloma cell proliferation and survival. (A) Percent green fluorescent protein-positive (%GFP+) cells after transduction with GFP-encod- ing, ZBP1-targeting shRNA1 (sh1), shRNA2 (sh2), shRNA3 (sh3) or appropriate scrambled control (scr) lentiviral constructs in U266 and H929 cells. All the time points were normalized to day 3 %GFP expression levels for each shRNA or scr control shown. (n=3). (B) %GFP+ cells after transduction with ZBP1-targeting sh1, sh2 or appropriate scr control lentiviral constructs in dexamethasone-sensitive MM.1S and its resistant derivative MM.1R cell line. All the time points were normalized to day 3 %GFP expression levels for each shRNA or scr control shown (n=3). (C) %GFP+ cells after the transduction of K562 and HeLa cells, which do not express ZBP1, with ZBP1-targeting sh1 or sh2 or appropriate scr control. The %GFP+ cells were normalized to day 3 %GFP expression levels for all the time points for each shRNA or scr control shown (n=3). (D, E) Photographs of tumors explanted at sacrifice from control, i.e., non-doxycycline (dox)-treated or dox-treated animals engraft- ed with H929 myeloma cells transduced with dox-inducible sh1 or sh2 targeting ZBP1 (D). Subcutaneous tumor volume of H929 or MM.1S over a period of up to 4
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