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Letters to the Editor
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Figure 2. Somatic mutations identified in CD8+ T cells of HTLV-2 positive subjects. (A) Mutation landscape in CD8+ T cells of human T-cell leukemia virus type 2 (HTLV-2) positive subjects (n=28). Coding variants identified by immunogene panel sequencing are presented, together with age, sex, number of mutations and mutation types. * (white asterisk), STAT3 variants detected in the deep amplicon sequencing but filtered out in the immunogene panel sequencing due to lower coverage but confirmed with visual inspection with the Integrative Genomics Viewer (Broad Institute, USA). The average expression of mutated genes in healthy controls are shown on the right, presented as counts per millions reads mapped (CPM) with mean ± standard deviation (n =5). The complete list of vari- ants and variant allele frequencies (VAF) can be found in the Online Supplementary Table S1. (B) Correlation plot of age vs. mutation number in HTLV-2 positive blood donors. (C) Percentages of somatic base substitutions and indel identified by immunogene panel sequencing in CD8+ T cells of HTLV-2 positive blood donors. (D) Normalized weights of COSMIC signatures contributions. Signature 1 (weight: 0.362) was highly related signature.
Daehong Kim,1,2 Mikko Myllymäki,1,2 Matti Kankainen1-4 Timo Järvinen,1,2 Giljun Park,1,2 Roberta Bruhn,5,6 Edward L. Murphy5,6 and Satu Mustjoki1,2,4
1Hematology Research Unit Helsinki, Helsinki University Hospital Comprehensive Cancer Center, Helsinki, Finland; 2Translational Immunology Research Program and Department of Clinical Chemistry and Hematology, University of Helsinki, Helsinki, Finland; 3Department of Medical and Clinical Genetics, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; 4iCAN Digital
Precision Cancer Medicine Flagship, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; 5Vitalant Research Institute, San Francisco, CA, USA and 6University of California San Francisco, San Francisco, USA
Correspondence:
SATU MUSTJOKI - satu.mustjoki@helsinki.fi doi:10.3324/haematol.2021.279140
Received: May 10, 2021.
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