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The favorable storability of bThal+ RBC
Figure 3E and F, respectively. Of note, several of the metabolic differences between the two groups at base- line were further exacerbated during storage, with signif- icantly lower levels of IMP, hypoxanthine and adenosine monophosphate (AMP) in the stored bThal+ RBC (Figure 5A). On the other hand, the bThal+ RBC had higher lev- els of urate throughout storage. Since human RBC lack a functional uricase, chemical oxidation of urate promotes further catabolism to allantoin and allantoate, that were found significantly lower and higher in the bThal+ RBC, respectively, throughout storage (Figure 5A). These
A
observations were accompanied by a higher degree of glutamine consumption and glutamate generation in bThal+ RBC after storage day 7 (Figure 5A; Online Supplementary Figure S8, respectively), suggestive of ongoing glutaminolysis. Interestingly, metabolites of purine metabolism such as urate, allantoate and gluta- mine represent very good predictors of bThal+ status in stored RBC (ROC curve analysis, Online Supplementary Figure S1C). Intertwined with purine (especially adeno- sine) metabolism, S-adenosylmethionine (SAM) synthe- sis and consumption were not significantly altered in
BC
Figure 1. Variation in hemolysis variables of fresh and stored red blood cells in beta thalassemia minor and control donors. (A) Time course evaluation of storage, osmotic, mechanical and oxidative hemolysis. Dashed lines represent trend lines of exponential or logarithmic models for storage and osmotic hemolysis, respec- tively. (B) Potassium leakage. (C) Storage levels of selected proteins related to osmotic/mechanical hemolysis. Data are presented as mean ± standard deviation. *P<0.05; F: fresh blood; A.U.: arbitrary units; bThal+: beta thalassemia minor.
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