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Frequent RUNX1 mutations in acute leukemia + pDC
33%); and less frequently CD15/65 (two cases) or myeloperoxidase (case case). Monocyte lineage markers were only expressed on blasts of M4/5-AML patients. T-cell markers were expressed in two cases (CD7 +/- CD5: patients N2 and N20) and the B-cell marker CD22 in two
other cases (N7 and N14) which was not sufficient to meet the definition of mixed-phenotype acute leukemia, as CD3/cCD3 or CD19/cCD79a were not expressed (Figure 2). CD123 was expressed in 12 cases of 15 (80%) at low level (MFIR: 15.58 [range, 2.5-63.1], MFI: 2,309 [range 465-
AB
CD
Figure 3. Expression of CD123 and cTCL1 on plasmacytoid dendritic cells from plasmacytoid dendritic cell-acute myeloid leukemia. (A) Comparison of mean fluo- rescence intensity ratio (MFIR) of CD123 between plasmacytoid dendritic cells (pDC) and immature CD34+ blasts in pDC-acute myeloid leukemia (pDC-AML). (B) Comparison of MFI of CD123 between pDC and immature CD34+ blasts in pDC-AML. (C) Comparison of MFIR of cTCL1 between pDC from pDC-AML, blastic pDC neo- plasms (BPDCN) and non-neoplastic pDC from healthy donors. (D) Comparison of MFI of cTCL1 between pDC from pDC-AML, BPDCN and non-neoplastic pDC from healthy donors. P-values (unpaired Mann-Whitney test) are marked above.
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