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Letters to the Editor
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Figure 3. MOLM-1 single clones harboring the deletion of breakpoint-associated retroelements exhibit no phenotypic changes but show reduced EVI1 expres- sion. (A) Illustration of the CRISPR-Cas9 mediated segmental deletion surrounding the breakpoint on the rearranged allele of chromosome 3 in MOLM-1 (left) and UCSD-AML1 (right). The deleted regions (MOLM-1: purple, UCSD-AML1: blue) encompass a MIR3 SINE element in MOLM-1, and representatives of all three RE subclasses in UCSD-AML1. Normalized proliferation (B) of deletion (del), nontargeting control (NTC) clones, NTC bulk of cells and wild-type (WT) cell line. (C) Quantitative polymerase chain reaction analysis of EVI1 mRNA levels (top panel) and representative western blot (bottom panel) of the full-length EVI1 isoform from samples shown in (B). Data shown in (B) and (C, top panel) are mean ± standard deviation from three independent experiments. Conservation across ver- tebrate genomes is taken from phastCons.13 SINE: short interspersed nuclear elements; LINE: long interspersed nuclear elements; LTR: long terminal repeats.
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