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expression of the ISC marker genes Lgr5 and Pleckstrin homology-like domain family A member 1 (Phlda1) and of the goblet cell marker Muc2 (Figure 6D). In contrast, no changes in the expression of Defa1 and Defa4 or Chga could be identified (Online Supplementary Figure S6A and B). In order to confirm the protection of ISC upon administration
of TUDCA, we utilized B6.129P2-Lgr5tm1(cre/ERT2)Cle/J mice which express green fluorescent protein (GFP) under con- trol of the Lgr5 gene promoter. On day 14 after transplanta- tion, we detected increased numbers of GFP+ cells in the small intestine of TUDCA-treated animals compared to vehicle-treated controls (Figure 6E and F). Previous studies
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Figure 4. Legend on next page.
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haematologica | 2021; 106(8)
