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anti-RhD antibodies modulate NK cell activity
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Figure 2. Specific anti-RhD anti- bodies mediate the degranula- tion of natural killer cells. (A to D) Preparation of the "unbound" antibody fractions and their effect on natural killer (NK)-cell degranulation. (A) Illustration of the production of unbound anti- RhD antibodies. Anti-RhD anti- bodies were incubated with RhD- (B) or RhD+ (C) erythrocytes and then the supernatant was col- lected and used for staining RhD- (left) and RhD+ (right) ery- throcytes. (D) Degranulation of NK cells incubated with the unbound antibody fractions, pre- pared as illustrated in (A). The NK-cell degranulation level was normalized to the basal percent of CD107a+ NK cells. (E to H) Preparation of the "bound" anti- body fractions and their effect on NK-cell degranulation. (E) Illustration of the production of bound anti-RhD antibodies. Anti- RhD antibodies were incubated with RhD- (F) or RhD+ (G) erythro- cytes. The eluates containing the bound antibody fractions were then extracted and used for staining RhD- (left) and RhD+ (right) erythrocytes. (H) Degranulation of NK cells incu- bated with the bound antibody fractions, prepared as illustrated in (E), and normalized as in (D). For all experiments, one repre- sentative experiment is shown out of at least two performed. ***P<0.001; Student's t-test. Error bars represent standard deviation of triplicates.
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haematologica | 2021; 106(7)
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