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Mobilization regulated by PPARd
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Figure 4. Regulation of granulocyte colony-stimulating factor-induced mobilization by PPARd signaling. (A, B) Mobilization efficiency modulated by the PPARd ago- nist (GW501516) of (A) white blood cells (WBC), lineage-Sca-1+c-kit+ (LSK) cells, and colony-forming units in culture (CFU-C) in the blood (n=8 for the group treated with phosphate-buffered saline [PBS]/bovine serum albumin [BSA] and n=11 for the group treated with granulocyte colony-stimulating factor (G-CSF) and (B) mRNA expression of Cpt1α and Angptl4 in bone marrow (BM) cells (n=8). (C, D) Mobilization efficiency modulated by the PPARd antagonist (GSK3787) in (C) WBC, LSK, and CFU-C in the blood (n=8-9) and (D) mRNA expression of Cpt1α and Angptl4 in BM cells (n=4-6). (E, F) Mobilization efficiency in BM PPARd+/- chimeric mice in (E) WBC, LSK, and CFU-C in the blood (n=8-11) and (F) mRNA expression of Cpt1α and Angptl4 in BM cells (n=6-8). (G) Modulation of mobilization (WBC, LSK, and CFU- C) by the PPARd agonist (GW501516) in mice fed with a normal diet (ND) or a fat-free diet (FFD) (n=4-5). Combined data from at least three independent experiments are shown. Data are mean ± standard error of mean. *P<0.05, **P<0.01, ***P<0.001 (Student t test, analysis of variance, and Mann-Whitney U test).
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