Page 176 - 2021_06-Haematologica-web

T. Suzuki et al.
A
B
Figure 5. Lipid composition of whole bone marrow during mobilization. Analysis of lipid mediators in whole BM sampled at -20°C with 100% methanol by liquid chromatography and tandem mass spectroscopy after eight doses of phosphate-buffered saline (PBS)/bovine serum albumin (BSA) or granulocyte colony-stimulating factor (G-CSF) in mice fed a normal diet (ND) or fat-free diet (FFD) for representative (A) ω3-fatty acids and (B) ω6-fatty acids (n=3-9). Combined data from at least three independent experiments are shown. Data are mean ± standard error of mean. *P<0.05, **P<0.01, ***P<0.001 (analysis of variance). PUFA: polyunsaturated fatty acids; EPA: eicosapentaenoic acid; HEPE: hydroxyeicosapentaenoic acid; DHA: docosahexaenoic acid; HDHA: hydroxydocosahexaenoic acid; AA: arachidonic acid; PG: prostaglandin, HETE: hydroxy-eicosatetraenoic acid
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haematologica | 2021; 106(6)