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Effect of G-CSF on mouse HSC
AB
Figure 3. Repopulating activity of cultured HSC1 and HSC2 cells. (A) Percentages of CD45.1 cells derived from HSC1 cells are shown. Percentage of CD45.1 cells in the myeloid lineage, B-cell lineage, CD4 T-cell lineage, and CD8 T-cell lineage in the culture with stem cell factor (SCF) alone were greater than those of freshly iso- lated cells after secondary transplantation. Percentage of CD45.1 cells in the myeloid lineage and B-cell lineage in the culture with SCF + granulocyte colony-stimu- lating factor (G-CSF) or SCF + granulocyte/macrophage (GM)-CSF were greater than those of freshly isolated cells after secondary transplantation. Percentage of CD45.1 cells in the myeloid lineage, B-cell lineage, CD4 T-cell lineage, and CD8 T-cell lineage in the culture with SCF+ thrombopoietin (TPO) were greater than those of freshly isolated cells at early months after primary transplantation. (B) Percentages of CD45.1 cells derived from HSC2 cells are shown. Percentage of CD45.1 cells in the B-cell lineage of the culture with SCF or SCF + TPO was significantly smaller than that of freshly isolated cells. Dots represent % chimerism in myeloid lin- eage, B-cell lineage, CD4 T-cell lineage and CD8 T-cell lineage from individual mice at a time point after transplantation. The lines above dots indicate the time periods compared. The unpaired t-test with Welch’s correction was used for statistical analysis between freshly isolated cells and cells in different culture conditions. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
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