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Zebrafish Hax1-associated neutropenia
anticipate that our model will serve as a platform to iden- tify new avenues for developing tailored therapeutic strategies for patients with CN.
Disclosures
No conflicts of interest to disclose.
Contributions
LD and BB made initial observations; LD performed most of the experiments and contributed to the design of the work; NA performed experiments in the transgenic lines; AMD performed quantitative polymerase chain reaction, immunostaining and flu- orescence in situ hybridization analysis; KW and JS provided useful insights and interpreted the data. BB supervised and sup- ported the study and wrote the manuscript. All authors read and edited the manuscript.
Acknowledgments
The authors would like to thank Patrick Müller (Fredrich Miescher Laboratory, Tübingen) for providing the wild-type TE strain of zebrafish and transgenic lines, Jochen Wittbrodt (Center for Organismal Studies, Heidelberg University) for providing the Cas9 construct, Annisa Claasen and Christine Gottschalk for technical help, and the Institute of Medical Virology and Microbiology for continuous support in confocal microscopy.
Funding
The work in BB’s laboratory is supported by the Deutsche Forschungsgemeinschaft (BA 5766/3-1), Deutsche José Carreras Leukämie-Stiftung (DJCLS11 R/2018), and Wilhelm-Sander- Stiftung.
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