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Impact of GC reaction on self-/polyreactivity in PCNSL
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Figure 5. Expression of SNRPC in primary lymphoma of the central nervous system (PCNSL). (A) Nearly all CD20+ (FITC) tumor cells of PCNSL show a nuclear expres- sion of SNRPC (Cy3). (B) Intermingled with tumor cells of a PCNSL, single CD68+ (FITC) macrophages also express SNRPC (arrows, Cy3); additionally, there are also SNRPC-negative CD68+ macrophages (arrowheads). (C) Cortical NeuN+ (FITC) neurons express SNRPC (arrows, Cy3). (D) Strongly activated GFAP+ (FITC) reactive astro- cytes express SNRPC (arrows, Cy3). (E) In the white matter, some Olig2+ (FITC) oligodendrocytes express SNRPC (arrows, Cy3). (F) CD34+ (FITC) endothelial cells of neocortical cerebral blood vessels do not express SNRPC (Cy3). (G) mRNA expression of SNRPC recognized on the ProtoArray in the normal central nervous system (CNS) and PCNSL. SNRPC is downregulated CNS and upregulated in PCNSL samples. The line represents the SNRPC tag on the U95Av2 microarray (Affymetrix) in individual samples of ten normal CNS tissues and 21 PCNSL published previously.15 SNRPC was significantly differentially expressed between CNS and PCNSL (P<0.01, Student t-test). (A-F) Double immunofluorescence with rabbit anti-SNRPC (clone EPR16034, Abcam, Cy3) and mouse anti-CD20 (clone L26, DCS, FITC in A), mouse anti-CD68 (clone KP1, DCS, FITC in B), mouse anti-NeuN (clone A60, Millipore, FITC in C), mouse anti-GFAP (clone GA-5, Biogenex, FITC in D), mouse anti- Olig2 (clone Olig2/2400, Abcam, FITC in E), and mouse anti-CD34 (cloneQBend/10, Biogenex, FITC in F). Microphotographs were taken with an Axiophot (Zeiss, Jena, Germany) and Zen 2 software (Zeiss). Original magnification: x400 (objective: x40). Inserts: x750 (A-C), x1000 (D-F). Overlay of the microphotographs and adjustment for contrast and brightness were performed with Adobe Photoshop software version CC.
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