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sCADM1 as a new diagnostic marker of ATLL
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Figure 1. High expression of sCADM1 transcript in adult T-cell leukemia/lymphoma. (A) Schematic representation of the structure and domain organization of mem- brane-bound cell adhesion molecule 1 CADM1 (mbCADM1) and its soluble form (sCADM1). mbCADM1 contains an extracellular domain (exons 2-10) containing three immunoglobulin-like C2-type domains, a transmembrane domain (exon 11), and a cytoplasmic domain (exon 12). sCADM1 is generated by alternative splicing in which intron 7 is retained and an in-frame stop codon is found immediately downstream of the immunoglobulin-like domain. Arrows indicate the position and direc- tion of primers used in RT-PCR reactions. The mbCADM1, sCADM1, and total CADM1 (tCADM1) transcripts were amplified using primers for exon 7 and exon 8, exon 6 and intron 7, and exon 4 and exon 5, respectively. Numbers indicate exon numbers of the CADM1 gene. (B) Quantitative RT-PCR analysis of tCADM1, mbCADM1, and sCADM1 expression in CD4+ T lymphocytes from healthy volunteers (n=5) and sorted CADM1+ cells from peripheral blood of patients with smoldering-type (n=6), chronic-type (n=6), and acute-type (n=7) ATLL. The data were normalized to β-actin gene expression and expressed relative to a healthy control sample. Mean ± stan- dard deviation is shown, **P<0.01 (Mann-Whitney U test).
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