Page 48 - Haematologica Atlas of Hematologic Cytology

ABC
DEF
GHI
Figure 4 Plasmodium malariae malariae in in in in thin blood smears (A) Ring-form trophozoites P P P malariae malariae rings are are characterized by a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a thicker cytoplasm cytoplasm than P P P P falciparum They show one chroma n n n n n n n n n n dot rarely two So-called “bird s s s s s s s s s s s s s s eye forms” may occasionally be seen (B) As they mature P P malariae trophozoites develop a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a more compact compact cytoplasm cytoplasm cytoplasm a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a rounded or or streaky chroma chroma n n n n n n n n n n n n n and and and and and a a a a a a a a a a a a a a a a a a a a a a a a a a a a a coarse coarse peripheral peripheral pigment pigment Note the the the the the the expanded compact compact cytoplasm cytoplasm the the the the the the large coarse coarse chroma chroma n n n n n n n n n n n n n n n n dots and and and and and the the the the the brown peripheral peripheral pigment pigment (C and and and and and D) As they develop P P malariae trophozoites expand expand their cytoplasm which appears compact with no vacuole Chroma n n n n n n n n n n n n n n n n n n n may have a a a a a a a a a a a a a a a a a a a a a a a a less de de de nite outline Pigment granules become larger and and and tend to to locate peripherally Note Note in in in in C C the the the the the peripheral peripheral peripheral distribu on on of of pigment pigment and and and the the the the the poorly de de de ned chroma n n n n n n n n n n n n n n n n n n outline Note Note in in in in in D the the the the the the the the the peripheral peripheral peripheral dense pigment pigment and and and and the the the the the the the the the elongated shape of of the the the the the the the the the upper red blood cell tropho- zoite zoite (E and and and F) As they develop further P malariae trophozoites may may elongate elongate across the the the the the the the the infected RBCs shaping the the the the the the the the so-called so-called “band-form” They may may also assume an an an an an oval shape with a a a a a a a a a a a a a a a a a a a a a a a a a a a vacuole forming the the the the the the so-called so-called “basket-form” (see the the upper infected red blood blood cell in in in in in in panel E) (G and and and H) P malariae schizonts These are frequently found in in in in in in peripheral blood blood smears They can contain 6-12 merozoites (more usually 8-10) and and have large nuclei surrounding a a a a a a a a a a a a a a a a a a a a a a coarse mass mass of of dark-brown dark-brown pigment pigment pigment The The infected cell may be completely lled (G) Note the the dark-brown dark-brown pigment pigment pigment mass mass located peripherally (H) Six merozoites clustered around a a a a a a a a a a a a a a a a a a a a a a quan ty of of coarse dense pigment pigment pigment (the “rose e” pa ern) (I) P malariae gametocytes These are rounded or or oval-shaped Infected cells have a a a a a a a a a a a a a a a a a a a a a a a a normal some mes reduced size and and tend to to to to to to be completely lled by the the parasite parasite Gametocyte cytoplasm cytoplasm is is pale-blue and and chroma n n n n n n n n n n n n is is pink to to to to to to red red red Brown pigment granules are sca ered inside the the parasite parasite cytoplasm cytoplasm 35