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Atypical TG2 expression activates the NF-κB pathway
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Figure 5. Atypical expression levels of tissue transglutaminase (TG2) drives inflammatory cytokine expression. In the cell culture, supernatant-secreted chemokines and cytokines were quantified by ELISA-based fluorescent detection cytokine array (RayBiotech Human Cytokine Array). Figures represent the mean±Standard Deviation of secreted protein levels from 3 independent experiments. (A) TG2-quantity-dependent-regulated nuclear factor kappa (κ)-light-chain- enhancer of the activated B-cell (NF-κB) transcription factor target genes, and TG2 inhibitor, NC9-sensitive secretory proteins. (B) TG2-modulated NF-κB transcription factor target genes, and TG2 inhibitor, NC9-insensitive secretory proteins. (C) TG2-dependent and TG2 inhibitor, NC9-sensitive secretory proteins. (D) TG2-dependent and TG2 inhibitor, NC9-insensitive secretory proteins. Statistical significance was determined via the Student t-test; asterisks show the significant differences com- pared to the wild type (Student t-test; *P<0.05, **P<0.01, ***P<0.005 ****P<0.001).
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