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K. Jambrovics et al.
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Figure 6. Tissue transglutaminase (TG2) both induces and guides nuclear translocation of nuclear factor kappa (κ)-light-chain-enhancer of the activated B-cell (NFκB). (A) Relative mRNA expression of TG2 in 1 mM all-trans retinoic acid (ATRA) + 30 mM NC9 treated NB4-WT, TG2-C, TG2-KD, TG2-ha, and TG2-KO cells measured at the indicated days by real-time Q-PCR and normalized to GAPDH mRNA expression (n=3). (B) Representative Western blot showing TG2 protein expression levels upon ATRA + NC9 treatment through 11 days (n=3). (C1-2) Representative Western blots showing total cell lysate samples TG2, p65/RelA, phospho-p65/RelA, and p50 protein expression levels in NB4 cell lines and its densitometric analysis, where graphs show the representation of the mean±Standard Deviation values of inte- grated density (n=6). (D1-2) Western blots of total cell lysate, cytosolic and nuclear fractions of TG2, and p65/RelA, phospho-p65/RelA, and p50 protein expression and its densitometry, respectively (n=10). (E1-2) Western blot analysis of NB4-WT cells upon ATRA, ATRA + NC9, and 5 mM MG132 treatment (n=6). Statistical sig- nificance was determined via the two-way analysis of variance (ANOVA; Bonferroni post-hoc test; *P<0.05, **P<0.01, ***P<0.005 ****P<0.001).
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haematologica | 2019; 104(3)