Page 135 - 2020_08-Haematologica-web

mTORi unlocks AML resistance to LSD1i
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Kasumi 72 h B
THP1 72 h
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Figure 4. Insulin receptor substrate 1 is transcriptionally upregulated in resist- ant acute myeloid leukemia cells following LSD1 inhibition. (A) Venn diagram illustrating the number of differentially as well as commonly expressed genes in THP-1 and KASUMI-1 cells 72 hours (h) following their treatment with DDP38003 (0.5 mM). (B) RNA sequencing tracks of insulin receptor substrate 1 (IRS1) gene following the indicated time points of treating KASUMI-1 and THP-1 cells with either vehicle or DDP38003 (0.5 mM). (C-D) Normalized IRS1 mRNA levels assessed in THP-1 (C) and OCI-AML3 (D) cells following their treatment with either vehicle (Veh) or DDP38003 (DDP, 0.5 mM) and using real-time quan- titative PCR (RT-qPCR). Data were statistically analyzed using either Student’s t- test *: P<0.05. (E-F) Normalized IRS1 mRNA levels assessed in THP-1 (E) and OCI-AML3 (F) cells transduced with the indicated short hairpin RNA (shRNA)
haematologica | 2020; 105(8)
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