Page 282 - Haematologica - Vol. 105 n. 6 - June 2020

M. Salzmann et al.
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Figure 1. Depletion of megakaryocytes and platelets with diphtheria toxin. (A) Genetic scheme of PF4-iCre iDTR mice, termed iDTRPlt mice. (B) Graphical overview of diphtheria toxin (DT) administration scheme. (C) Femora of untreated and mice treated for four and seven days with DT. Bone marrow was stained for endothelial cells (endomucin, red) and megakaryocytes (CD41, green). Scale bar: 200 μm. (D) Platelet counts and (E) mean platelet volume after indicated days of DT treatment. (F) Linear regression of decrease in platelet counts of iDTRPlt mice within first four days of DT treatment. 95% confidence interval of X-intercept: 4.563-5.318. (D-F) Curves of groups were compared to that of iDTRPlt + DT (n=3-8). (G) Titer of anti-DT IgG after indicated times of treatment, normalized to a DT-boosted mouse (n=4- 17). (H) Leukocyte counts and (I) body weight after indicated days of DT treatment. (H-I) Curves of groups were compared to that of iDTRPlt + DT (n=3-8).
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haematologica | 2020; 105(6)
Platelets/μL (x103)
Platelets/μL (x103)