Page 163 - Haematologica April 2020
P. 163
CD99 overexpression in AML
AB
D
E
C
F
Figure 4. Effect of ectopic expression of CD99 long isoform on reactive oxygen species (ROS) lev- els, DNA damage and cell apoptosis. (A and B) ROS levels of EV, CD99-L and CD99-S cells deter- mine by flow cytometry using the cell ROX reagent in THP-1 cells (n=4) measured by flow cytome- try. (C) Western blot analysis of H2Axγ in THP-1 EV, CD99-L and CD99-S. (D and E) Apoptosis analy- sis of EV, CD99-L and CD99-S cells determined by annexin V+ cells through flow cytometry in THP- 1, U937 and MOLM-13 cells (n=4). (F) Western blot analysis of cleaved caspase-3 in THP-1 EV, CD99-L, and CD99-S. (**** P<0.0001; ** P<0.01).
haematologica | 2020; 105(4)
1005
