Page 112 - Haematologica Atlas of Hematologic Cytology

Sudan black detect myeloid cells by staining cytoplasmic granular contents and better identify Auer rods whe- reas monocytic cells can be be better identified with the non-specific esterase reaction Finding at least 3% of bla- sts positive for Sudan black or or peroxidase distinguishes AML from acute lymphoblastic leukemia Immunophe- notyping is is essential in in conditions not not identifiable on on on the basis of morphological and cytochemical criteria such as as as acute myeloid leukemia leukemia with with minimal differentiation differentiation some monoblastic leukemias with with poor differentiation differentiation pure erythroid leukemia leukemia leukemia acute acute megakaryoblastic leukemia leukemia leukemia and acute acute basophilic leukemia leukemia leukemia The antigenic markers useful for the identification of myeloid blasts are listed in Table 2 Table 2 n n n n genic mar ers useful for iden ca on of myeloid cells * nde ned cluster The current reference classification classification of AML is the 2001 WHO classification classification up-dated in 2008 and 2016 (Vardi-
man et et al al 2002 2009)(Arber et et al al 2016) (Table 3) Table 3 orld Health rganiza on on classi ca on on of acute myeloid leu emia and related precursor neoplasms
cute cute myeloid myeloid leu leu emia emia with with recurrent gene c c c c abnormali es Acute Acute myeloid myeloid myeloid leukemia leukemia with with with t(8 21)(q22 q22 q22 q22 q22 1) 1) RUNX1-RUNX1T1*
Acute Acute Acute myeloid myeloid leukemia leukemia leukemia with with with inv(16)(p13 1q22) or t(16 16)(p13 16)(p13 1 1 1 1 1 1 1 1 1 1 1 1 1 1 q22) q22) CBFB-MYH11* Acute Acute Acute promyelocy c c c c c leukemia leukemia leukemia with with with PML-RARA*
Acute Acute Acute myeloid myeloid leukemia leukemia leukemia with with with t(9 11)(p21 3 3 3 3 3 3 3 3 3 q23 3) 3) 3) KMT2A-MLLT3
Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia with with with inv(3)(q21 3q26 2) 2) 2) or t(3 3)(q21 3)(q21 3 3 3 3 3 3 3 3 3 3 3 3 q26 q26 2) 2) 2) GATA2 MECOM Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia (megakaryoblas c) with with with t(1 22)(p13 3 3 3 3 3 3 3 3 q13 1) RBM15-MKL1 Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia with with BCR-ABL1
Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia with with with gene muta muta ons**
Acute myeloid myeloid leukemia leukemia with with mutated NPM1
c c c c e e e e e e e e e e e e e e e m m m m m m m m m eloi eloi eloi le le le le emia emia emia i i i i i i i i i i i iallelic m m m m m m m m m a a a a a a a o o o o o o o o o o Acute myeloid myeloid leukemia with with mutated RUNX1 cute cute myeloid myeloid myeloid leu leu emia emia with with myelodysplasia related related changes
Therapy related myeloid myeloid myeloid neoplasms
Acute Acute myeloid myeloid myeloid leukemia leukemia NOS
Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia with with minimal di eren a a a a a a a on on Acute Acute Acute myeloid myeloid myeloid leukemia leukemia leukemia without matura matura on on Acute Acute Acute myeloid myeloid leukemia leukemia leukemia with with matura matura on on Acute Acute Acute myelomonocy c c c c c c c leukemia leukemia Acute Acute monoblas c c c c c c c and monocy monocy c c c c c c c leukemia leukemia leukemia Pure erythroid leukemia Acute Acute megakaryoblas c c c c leukemia leukemia leukemia Acute Acute Acute basophilic leukemia leukemia Acute Acute panmyelosis with myelo myelo brosis
Myeloid Myeloid sarcoma
Myeloid Myeloid prolifera ons associated associated with with Down Down syndrome
Transient abnormal myelopoiesis associated associated associated with with with Down Down Down syndrome
syndrome
syndrome
Myeloid leukemia associated associated with with Down Down syndrome
NOS: not otherwise speci ed ed *Cases with with this gene c c c c c c c abnormality are considered to to be acute leukemias without regard to to blast count **These en en es es es es have no no no dis nc ve ve morphological features
Speci city
luster of di eren a on Panmyeloid
CD13 CD33 CD117 myeloperoxidase*
Myeloid mature cells CD15 CD11b
Monocy c c c cells CD11b
CD11c CD14 CD64 lysozyme*
Erythroid cells CD71 glycophorin glycophorin A (CD235a) glycophorin glycophorin C C C C (CD236R)
Mega aryocy c c c cells CD41 CD61
Progenitor cells CD34 terminal deoxynucleo dyl transferase*
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