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p66shc deletion exacerbates leukemia in TCL1 mice
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Figure 5. p66Shc deficiency in leukemic cells results in enhanced expression of homing receptors and reduced expression of the egress receptor S1PR1. (A-E) Quantitative real-time polymerase chain reaction analysis of the mRNA levels (left) and flow cytometric analysis of surface expression (right) of CXCR4 (A), CCR7 (B), S1PR1 (C), CCR2 (D) and CXCR3 (E) in CD5+CD19+ cells purified from either wildtype (WT) (n=16) or p66Shc-/- (n=15) mice (B1a cells) and from Eμ-TCL1 (n≥16) or Eμ-TCL1/p66Shc-/- (n≥16) mice with overt leukemia. The relative gene transcript abundance was determined on triplicate samples using the DDCt method. Representative flow cytometric plots of the indicated stains are shown on the right. Chemotaxis toward the respective chemokines is shown in Online Supplementary Figure S6. Mean ± standard deviation. One-way analysis of variance (ANOVA), multiple comparisons. ****P≤0.0001; ***P≤0.001; **P≤0.01; *P≤0.05.
haematologica | 2019; 104(10)
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