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New Drosophila model for chronic myeloid leukemia
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Figure 2. BCR-ABL1 expression affects ommatidia development by altering the dAbl signaling pathway. (A) Adult eyes showing the phenotypic classes used to quantify the severity of the vari- able phenotype due to BCR-ABL1 expression in eye cells committed to ter- minal differentiation. Posterior is on the left. Class 0 (green) corresponds to the average phenotype shown by gmrGal4, UAS-BCR-ABL1 4M flies; the ommatidia are almost totally absent, the eye depig- mented region is very small and the eye appears flatter than that of wild-type eyes. Class -1 (pale blue) corresponds to more severe phenotypes: the eyes are even flatter than class 0 eyes and the depigmented area is enlarged including a dorso-ventral sector in the most posterior region of the eye. Class +1 (pink) corresponds to less severe phenotypes: a few ommatidia are visi- ble, the eyes are more bulging and the depigmented area is absent indicating better eye cell differentiation. (B-F) Adult eyes from flies of the indicated genotypes were classified to evaluate the frequency of the three phenotypic classes. (B) A piled histogram chart showing the frequencies of the different phenotypic classes in flies expressing BCR-ABL1 (gmrGal4,4M/+), co-express- ing BCR-ABL1 and the EGFP (UAS- EGFP/+;gmrGal4,4M/+), expressing BCR-ABL1 but having a partial loss of the endogenous Abl gene through the
EF
mutation (Abl /+;gmrGal4,4M/+), RNAi targeting Abl (Abl-RNAi/+;gmrGal4,4M/+), expression of a dominant negative dAbl mutant (UAS-AblK417N;gmrGal4,4M/+) or overexpression of the wild-type Abl pro- tein (UAS-Abl;gmrGal4,4M/+). (C-F) Piled histogram charts showing the fre- quencies of the three phenotypic class- es in flies expressing BCR-ABL1 (gmrGal4,4M) and heterozygous for a loss of function allele or for a deletion of genes that behave as genetic modifiers of the embryonic lethality due to Abl LOF: prospero (C), failed axon connec- tion (D), Disabled (E) and enabled (F). The statistical comparisons were con- ducted using a Mann-Whitney test
heterozygous Abl1 1
(*P<0.05, **P<0.01, ***P<0.001).
haematologica | 2019; 104(4)
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