Page 134 - Haematologica Vol. 109 - July 2024

ARTICLE - Ppm1b regulates HSC homeostasis
Z. Lu et al.
from Ppm1bCKO BM were also significantly reduced in primary and subsequent recipients (Figure 3F, Online Supplementary Figure S3J). In addition, B cells derived from Ppm1bCKO BM also showed a progressive decrease in the recipients (Online Supplementary Figure S3K, L), which is consistent with the defect of B cells in primary CKO mice.
Ppm1b regulated the hematopoietic stem cell pool and B-cell differentiation in adult hematopoiesis
Given that the defects of Ppm1bCKO mice may be due to a consequence of embryo stage, we analyzed whether Ppm1b also play roles in adult hematopoiesis. To this end, WT mice were pretreated with Ppm1b inhibitor HN252 and then challenged with a sublethal dose of 5-FU (Figure
AB
CD
EF
Figure 2. Ppm1b deficiency mice exhibited B-cell leukocytopenia. (A) Absolute number of white blood cells (WBC) and lympho- cytes in peripheral blood from Ppm1bCKO and Ppm1bfl/fl mice at the age of 8 weeks (N=14). (B) Statistical analysis of the percentage of B cells in bone marrow (BM) from indicated mice (N=6). (C) Representative flow cytometric profiles of the strategy for common lymphoid progenitors (CLP), Lin−Sca-1lowc-KitlowCD127+ cells. (D) Quantification of the cell number of CLP cells in BM from indicat- ed mice (N=4). (E) Representative profiles of the colonies after 7 days of culture from indicated mice. (F) Quantification of the colony number in (I) (N=3). All P values were determined by unpaired two-tailed Student t test. See also Online Supplementary Figure S2.
Haematologica | 109 July 2024
2148