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M. Krevvata et al.
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BCD
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Figure 5. Myelodysplastic syndrome (MDS) cell engraftment is not affected by mesenchymal stem cells (MSC). (A) Representative phenotypical characterization of the ex vivo-expanded human MSC used in the transplantation experiments. Trilineage in vitro differentiation of ex vivo expand- ed MDS patient MSC into (B) osteoblasts, (C)
adipocytes, and
Differentiated, (2) Undifferentiated, (3) Positive Control] (E) Bone marrow human mononuclear cells were intrafemorally transplanted into NSG-S mice alone or in combination with ex vivo-expand- ed MSC isolated from various origins. Percentages of hCD45+ cells present in the murine bone mar- row at 6-8 weeks post transplantation. Red line at 0.1% represents the positive engraftment thresh- old level. Comparisons showed no significant sta- tistical differences between the four groups. (F) NSG mice intrafemorally transplanted with labeled MSC-green fluorescent protein-luciferase. Bioluminescence levels were measured in vivo at the timepoints indicated.
(D) chondrocytes. [(1)
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