Page 273 - Haematologica Vol. 107 - September 2022

LETTER TO THE EDITOR
ency, we transduced STAG2 KO human HSPC with two in- dependent short hairpin RNA (shRNA) targeting STAG1 as well scrambled (Scr) control shRNA. The pLKO shRNA vec- tors were engineered to express Kusabira orange (KuO) en-
abling tracking of the transduced cells in conjunction with the eGFP marker for STAG2 null cells. Both STAG1 shRNA showed successful knockdown of STAG1 at the mRNA and protein level 72 hours post-transduction (Online Supple-
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