Page 159 - Haematologica Vol. 107 - September 2022

ARTICLE - Use of aqueous humor in VRL management
X. Wang et al.
V4) were 90% (26/29) and 60% (10/16), respectively. Thus, those results suggest that AH can serve as a surrogate for mutational analysis of VRL and is more easily access- ible than VF biopsies.
In addition, to avoid unnecessary clinical injury to pa- tients with binocular disease, AH/VF sampling is usually
performed in a single eye. However, patient V5 under- went AH extraction from both eyes for sequencing. Over- all, 32 mutations were identified in AH samples from either the left or right eye, over 80% (26/32) of which were detected in both samples, with similar allele fre- quencies (R2=0.899) (Online Supplementary Figure S2).
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Figure 3. Dynamic profiling of circulating tumor DNA and interleukin-10 levels in aqueous humor or vitreous fluid samples. The treatment histories of five patients (identity numbers in the top right corner of each panel) with dynamic and sequential sampling of aqueous humor (AH) or vitreous fluid (VF) are shown at the bottom of each panel. The allele frequency changes to driver mu- tations and other alterations are shown in the line chart. Interleukin-10 levels (pg/mL) at each time point are indicated by the green circles at the top of each panel. AF: allele frequency; ctDNA: circulating tumor DNA; IL-10: interleukin.10; LEN: lenalidomide; TMZ: temozolomide; HD-MTX: high-dose methotrexate.
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