Page 125 - Haematologica May 2022

GECPAR, eRNA master regulator of germinal center
oplasm and cytoplasm of OCI-LY1, a cell line with 5-fold higher levels of chromatin-associated GECPAR than the other cell lines (Figure 1C). Semi-quantitative directional RT-PCR showed that chromatin association was particu- lar to GECPAR since its antisense transcript, when
A
expressed, was more ubiquitously distributed (Online Supplementary Figure S1B). Quantification of KCNQ1OT1, MALAT1 and β-actin mRNA served as a control for chro- matin-associated, nuclear and cytosolic RNA, respective- ly (Online Supplementary Figure S1C).
Figure 1. Figure continued on following page.
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