Page 129 - 2022_03-Haematologica-web

ZRSR2 & ZRSR1 co-operate in splicing of U12 introns
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Figure 3. Human and mouse ZRSR1 are located in non-orthologous genomic loci. (A) Levels of ZRSR1 and ZRSR2 transcripts in human whole blood and spleen cells. Expression data are collated from Genotype-Tissue Expression (GTEx) portal. (B) Expression levels of Zrsr1 and Zrsr2 transcripts in murine spleen cells (Bgee database) and myeloid precursors (in-house RNA sequencing of sorted CMP, GMP and MEP cells). TPM: transcripts per million reads. (C) Genomic location of human and murine ZRSR1 genes. Orange arrows denote the direction of transcription for each gene. (D) H3K4me3, H3K4me1 and H3K27ac ChIP-seq signals and regions of open chromatin at human and murine ZRSR1 locus. All data were downloaded from the ENCODE Consortium. Human ChIP-seq and DNase-seq data are for common myeloid progenitors, while murine data are from following sources - H3K4me3: CD1 embryonic erythroblasts; H3K4me1: CD1 embryonic megakaryocytes; H3K27ac: bone marrow macrophages; ATAC-seq: adult erythroblasts; CMP: common myeloid precursors; GMP: granulocyte monocyte precursors; MEP: megakaryocyte erythroid precursors.
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